Lab Testing & Safety Report

Dr. Nurten Abaci Kaplan Medically reviewed by Dr. Nurten Abaci Kaplan, PhD Pharmacognosy · Herbal Supplement Specialist The Proof · Lab Transparency

Independent Lab Testing & Safety Report

The complete Eurofins certificates of analysis for Natural Shilajit Resin, Lot 2232-132: heavy metals, microbiology, fulvic acid, and adulterant screening, in plain English, with the raw PDFs published in full.

Issued by Global Wellnest Distribution Inc. Lot 2232-132 12 min read
  • EurofinsISO/IEC 17025 lab
  • ICP-MSHeavy metals method
  • 3 COAsPublished in full
  • 34.52%Fulvic acid, dry weight
  • PASSAmazon certification
  • Jun 2026Last updated
Lab test tubes with resin samples at varying concentrations

TL;DR: What the lab reports actually show

  • Every heavy metal tested came in below its specification limit. Arsenic, the highest, used just 25.7% of the USP daily-exposure limit; mercury used 0.4%.
  • All pathogens were Absent across two testing rounds: E. coli, Salmonella, Staph aureus, and Pseudomonas, with yeast & mold < 100 CFU/g.
  • Fulvic acid measured 34.52% by dry weight, confirming a genuine humic mineral matrix, not a synthetic reconstruction.
  • A 28-compound PDE5 adulterant screen returned Not Detected, and the full Amazon certification panel returned PASS. All testing by Eurofins (ISO/IEC 17025).

Overview Scope of Testing & What These Reports Cover

This report presents the complete independent laboratory findings for Natural Shilajit Resin, Lot 2232-132, sourced from the Altai Mountains, with a plain-English interpretation of every result and how it compares to U.S. FDA, USP, and WHO benchmarks.

All testing was performed at Eurofins Food Chemistry Testing Madison, Inc., one of the world’s largest ISO/IEC 17025-accredited food and supplement testing networks. Methods follow the Official Methods of Analysis of AOAC INTERNATIONAL and USP current-revision chapters. Results are presented exactly as reported: no values have been modified, rounded, or omitted.

Reports referenced in this document

COA #5381929-0, Microbiology + Fulvic Acid (May 2026).  COA #5428992-1, Heavy Metals confirmation (June 2026). Both 2026 certificates are published in full below.

The Documents Certificates of Analysis, Published in Full

Below are the unredacted Eurofins certificates for Lot 2232-132, exactly as issued. Page through them in the reader, open them in a new tab, or download the original PDFs. Everything interpreted in the rest of this report is drawn directly from these pages.

PDF COA-5381929-0_Lot-2232-132_Microbiology-Fulvic.pdf Eurofins · 3 pages · Report 5381929-0 · May 2026
Loading certificate…
Issued by Eurofins Food Chemistry Testing Madison, Inc. Facility address redacted
PDF COA-5428992-1_Lot-2232-132_Heavy-Metals.pdf Eurofins · 1 page · Report 5428992-1 · June 2026
Loading certificate…
Issued by Eurofins Food Chemistry Testing Madison, Inc. Facility address redacted

Part I Heavy Metals Testing

1.1 · Why it mattersWhy heavy metals must be tested in a mineral product

Shilajit is a geological exudate: it forms over thousands of years from the compression and microbial decomposition of plant matter inside high-altitude rock. Because it originates in the earth, it can absorb trace elements from the surrounding geology, including measurable amounts of heavy metals. This is not unique to shilajit, arsenic, lead, cadmium, and mercury are routinely detected in rice, apple juice, leafy vegetables, seafood, and organic produce.

The question is never whether trace elements are present at all, in a mineral-origin product, trace amounts are expected. The relevant question is whether levels fall within the ranges toxicology considers safe for chronic daily exposure. The FDA, WHO, AHPA, and USP each publish Permitted Daily Exposure (PDE) limits that answer exactly this.

1.2 · MethodologyICP-MS via AOAC official methods

Heavy-metal analysis used Inductively Coupled Plasma Mass Spectrometry (ICP-MS), following AOAC Official Methods 2011.19, 993.14, and 2015.01. ICP-MS is the internationally recognised gold standard for trace-element detection in food and supplements: it atomises the sample in an argon plasma at roughly 6,000-10,000 °C, then separates elements by mass-to-charge ratio at parts-per-billion (ppb) precision. Published validation confirms recovery of 92.2-103.1% across As, Cd, Pb, and Hg matrices, with repeatability below 3.55%. The Madison facility holds accreditation number 2918.01 under the ILAC Mutual Recognition Arrangement.

1.3 · ResultsActual heavy-metal results, Lot 2232-132

Reported in COA #4545687-0, Elements by ICP Mass Spectrometry, serving size 300 mg:

Element Measured result Spec limit Result
Arsenic (As) 0.00193 mg/serving (1.93 µg) ≤ 0.01 mg PASS
Lead (Pb) 0.000324 mg/serving (0.324 µg) ≤ 0.01 mg PASS
Cadmium (Cd) 0.0000367 mg/serving (0.0367 µg) ≤ 0.0041 mg PASS
Mercury (Hg) 0.00000408 mg/serving (0.00408 µg) ≤ 0.002 mg PASS
Chromium (Cr) 0.000473 mg/serving (0.473 µg) ≤ 0.02 mg PASS

Source Eurofins COA #4545687-0, Lot 2232-132, tested June 2024. Method: ICP-MS per AOAC 2011.19, 993.14, 2015.01 (Modified).

All five elements tested below their specification limits. The safety margin is substantial: arsenic, the highest measured element, came in at 19.3% of its spec limit, leaving an 80.7% margin. Mercury registered at just 0.204% of its limit.

1.4 · In practiceWhat these values mean at maximum daily use

Assuming two servings daily (600 mg total) against USP <2232> Permitted Daily Exposure limits:

Element Daily exposure (2 servings) USP PDE limit % of limit
Arsenic 3.86 µg/day 15 µg/day 25.7%
Lead 0.648 µg/day 5 µg/day 13.0%
Cadmium 0.0734 µg/day 5 µg/day 1.5%
Mercury 0.00816 µg/day 2 µg/day 0.4%

Even at maximum daily use, no element approaches its regulatory daily-exposure threshold.

The humic matrix note

A 2024 analysis in Biological Trace Element Research (PMID 38393486) found that the fulvic and humic acids in shilajit exhibit documented chelation activity, binding trace metals through their carboxyl and phenolic groups. Even at the trace levels measured here, the elements exist within a biogeochemically active organic matrix rather than as free ionic contaminants.

1.5 · ContextHeavy metals in everyday foods

The FDA’s Total Diet Study found arsenic in 43% of all food samples tested, with individual seafood readings reaching 10,900 ppb. A Consumer Reports investigation of 45 fruit juices found measurable heavy metals in every single product, and organic products showed no advantage over conventional ones. A 300 mg serving of resin is a fraction of the food mass in a glass of juice or a bowl of rice, so absolute daily exposure from this product is demonstrably low by comparison.

Part II Microbiology Testing

Microbial contamination, pathogenic bacteria, mold, and yeast, is an independent safety dimension, distinct from heavy metals. Testing follows USP current-revision chapters 2021 and 2022. This product has been tested across two rounds, with the 2024 evaluation (COA #4545687-0) and the 2026 confirmation (COA #5381929-0) showing consistent results.

2.1 · May 2026COA #5381929-0, Eurofins Micro Lab, Madison

Test Method Result Status
Aerobic Plate Count USP Ch. 2021 8,600 CFU/g Within limits
Escherichia coli USP Ch. 2022 Absent / 10 g PASS
Salmonella USP Ch. 2022 Absent / 10 g PASS
Combined Yeast & Mold USP Ch. 2021 < 100 CFU/g PASS
APC Suitability USP Preparatory PASS Validated
E. coli Suitability USP Preparatory PASS Validated

2.2 · June 2024COA #4545687-0, extended pathogen panel

Test Result Specification Status
Aerobic Plate Count 6,700 CFU/g ≤ 10,000,000 CFU/g PASS
E. coli Absent /10 g N/A PASS
Enterobacteriaceae < 10 MPN/g ≤ 1,000 MPN/g PASS
Pseudomonas aeruginosa Absent /10 g N/A PASS
Salmonella Absent /10 g N/A PASS
Staphylococcus aureus Absent /10 g N/A PASS
Combined Yeast & Mold < 100 CFU/g ≤ 1,000,000 CFU/g PASS

What “suitability” means: before reporting a valid absence for E. coli or Salmonella, the lab must first confirm the test conditions can detect those organisms in this specific matrix. A PASS on both suitability tests means the absence result is a true negative, not a testing artifact. The critical finding, absence of all pathogens, is consistent across both testing periods.

Part III Fulvic Acid Content

COA #5381929-0 (May 2026) reports Fulvic Acid at 34.52 g/100g (34.52% by dry weight) for Lot 2232-132, measured by Eurofins Analytical Services India using the IRXNK fulvic acid method, a specialised extraction-based protocol.

Fulvic acid is the low-molecular-weight fraction of humic substances, distinguished from heavier humic acid by its solubility in both acid and alkaline conditions. It carries a high density of oxygen-containing functional groups (carboxyl, carbonyl, phenolic hydroxyl) that underlie its documented ability to bind minerals, transport nutrients across cell membranes, and participate in electron transfer. A result of 34.52% means slightly more than one-third of the resin’s dry mass is measurable fulvic acid, substantial for a natural mineral-origin product.

A purity marker, not a bioactivity score

Fulvic acid percentage is a compositional purity marker, not a direct measure of biological activity, which also depends on molecular-weight distribution and functional-group density. This is precisely why DBP (dibenzo-α-pyrone) testing is a separate, more specific layer of molecular authentication, DBPs form only through the geological humification that produces authentic shilajit and are absent from generic humic-acid extracts.

Part IV Adulterant Screening (Amazon Certification)

COA #4545687-0 includes a targeted LC-MS/MS screen for 28 sexual-enhancement adulterants, synthetic PDE5 inhibitors (sildenafil, tadalafil, vardenafil, and 25 analogs) occasionally found in illegally adulterated supplements.

Adulterant category Compounds screened Result
Sildenafil & analogs 14 compounds Not Detected
Tadalafil & analogs 8 compounds Not Detected
Vardenafil & analogs 6 compounds Not Detected
Overall certification 28 compounds total PASS

Reporting limit: 50 ppm per compound. The complete Amazon Certification panel, Adulterants, Contaminants, Pathogens, Microbiological, GMP, Content Claims, and Label Review, returned an Overall Certification: PASS.

Part V Testing Laboratory Credentials

All COAs were issued by Eurofins Food Chemistry Testing Madison, Inc., 6304 Ronald Reagan Ave, Madison, WI 53704, authorised by Edward Ladwig, President. Eurofins operates one of the world’s largest testing networks, over 900 laboratories across 50+ countries. The Madison facility holds accreditation number 2918.01 under the ILAC Mutual Recognition Arrangement, so its results are recognised by regulatory authorities across participating countries.

ISO/IEC 17025 accreditation requires:

  • External audits by an independent national accreditation body (A2LA in the U.S.)
  • Demonstrated proficiency through inter-laboratory comparison programs
  • Documented and validated analytical methods for each test performed
  • Measurement-uncertainty statements available on request
  • Results that carry evidentiary weight in regulatory enforcement

Chain of custody is documented across every COA header: Project IDs assigned by Global Wellnest Distribution Inc., Eurofins Sample IDs assigned independently by the lab, plus Receipt, Login, and Date-Started fields. The manufacturer did not conduct or oversee the testing.

Part VI Comprehensive Results Summary

Every parameter, its Lot 2232-132 result, the governing regulatory reference, and its real-world comparison context, on one page.

Category Parameter Result Regulatory reference
Heavy Metals Arsenic 0.00193 mg/serving ✓ USP PDE 15 µg/day
Heavy Metals Lead 0.000324 mg/serving ✓ USP PDE 5 µg/day
Heavy Metals Cadmium 0.0000367 mg/serving ✓ USP PDE 5 µg/day
Heavy Metals Mercury 0.00000408 mg/serving ✓ USP PDE 2 µg/day
Heavy Metals Chromium 0.000473 mg/serving ✓ ≤ 0.02 mg spec limit
Microbiology E. coli Absent /10 g ✓ USP: Absent
Microbiology Salmonella Absent /10 g ✓ USP: Absent
Microbiology Staph. aureus Absent /10 g ✓ USP: Absent
Microbiology Pseudomonas Absent /10 g ✓ USP: Absent
Microbiology Yeast & Mold < 100 CFU/g ✓ ≤ 1,000,000 CFU/g
Composition Fulvic Acid 34.52 g/100g ✓ Compositional marker
Adulterants 28 PDE5 inhibitors Not Detected ✓ Zero tolerance
Overall Amazon Certification PASS ✓ Full panel

Part VII DBP Verification & The Third Layer

Fulvic acid analysis establishes what is in the product. Heavy-metals testing establishes it is safe. DBP verification establishes it is authentic. Dibenzo-α-pyrones are oxygen-heterocyclic compounds produced exclusively through the geological humification and pressurisation that creates shilajit resin. Their presence, and specific molecular profile, is the most specific fingerprint available for distinguishing authentic resin from generic humic concentrates or reconstructed imitations.

Safety, cleanliness, identity, heavy metals, microbiology, and the DBP profile are the three pillars of the documentation system.

DBP testing for Lot 2232-132 is published in a separate report, forming the third pillar alongside the safety and cleanliness data above.

Verification · 5 stepsHow to verify your lot

Every jar carries a Lot Number printed on the label. The results here apply specifically to Lot 2232-132. To verify any lot:

  1. Match the Lot Number on your label to the COA header.
  2. Confirm the laboratory name, Eurofins Food Chemistry Testing Madison, Inc.
  3. Check the accreditation number, 2918.01 (ILAC MRA).
  4. Note the Receipt and Test-Start dates, which confirm the COA corresponds to actual batch testing.
  5. Review the numeric results, not just the pass/fail checkbox, but the measured values against the spec limits.

Full Certificates of Analysis for all active lots are published without redaction and available for download above.

FAQCommon Questions About the Lab Reports

Does shilajit naturally contain heavy metals?
Yes, in trace amounts, and that is expected for any mineral-origin product. What matters is the level. In Lot 2232-132, every element tested below its specification limit, with arsenic (the highest) using only 25.7% of the USP daily-exposure limit.
What does “Absent /10 g” mean for pathogens?
It means no organisms were detected in a 10-gram test portion, and the lab first validated (via a “suitability” test) that the method could detect them in this matrix, so the absence is a true negative, not a testing artifact.
Is 34.52% fulvic acid good?
It confirms a genuine humic mineral matrix, with over a third of the dry mass being measurable fulvic acid. Note that fulvic percentage is a purity marker, not a bioactivity score; any claim above 45% is not chemically possible in native resin.
Can I see the original certificates?
Yes. The unredacted Eurofins PDFs for Lot 2232-132 are embedded above, page through them, open in a new tab, or download the originals. Only the facility street address is redacted.
Who did the testing?
Eurofins Food Chemistry Testing Madison, Inc., an ISO/IEC 17025-accredited lab (accreditation 2918.01). The manufacturer did not conduct or oversee the testing.

* These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. This report reproduces quantitative results from Eurofins Certificates of Analysis for Lot 2232-132 without modification; it is provided for informational purposes and is not medical advice.